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台灣甲魚仙人掌桿菌群之基因型、表現型及毒力基因分析

為了解決Benz SL 2021的問題，作者鄭立吾 這樣論述：

Chinese softshell turtle (Pelodiscus sinensis), which belongs to the Testudines, Trionychidae, is one of the most prevalent aquaculture species in Taiwan. In natural habitats, Trionychidae family is mostly found in Asia, Africa, and North America. Therefore, it is very important to understand the g

enotypic and phenotypic characteristics of this pathogen. In our best understanding, information about genotype, phenotype, and pathogenicity characterization of Bacillus cereus group (Bcg) in Taiwan remains few. Totally 57 Bcg isolates from 2016 to 2019 obtained from different farm in Kaohsiung cit

y and Pingtung county were used to conduct by following experiment 1. Pulsed-field gel electrophoresis (PFGE) and Enterobacterial Repetitive Intergenic Consensus (ERIC) Polymerase Chain Reaction (PCR) when screening the bacterial genotype. 2. 16S rDNA and pyruvate carboxylase (pycA) gene as phylogen

etic marker to construct phylogenetic tree. 3. Phenotype bioassays using API-20E. 4. PCR detection of virulent genes and antibiotic susceptibility test. Result show strains majorly belong to single genotype (N4, S4 and E4), however, it still has different genotypes appear with only one strain each.

In phylogenetic tree reveal the major genotype groups were congregating in an independent group. Phenotype bioassays shows a few of differences between strains in each genotype. In virulent genes profile, cereulide synthetase gene (ces) and nonribosomal peptide synthetases (CER) was not identified i

n any of the isolates. By contrast only 1 (1/57, 1％) isolate contain cytotoxin K (cytK), 46 (46/57, 80％) isolates possessed the hemolysin BL, C subunit (hblC) and 52 (52/57, 91％) isolates possessed the nonhemolytic enterotoxin, A subunit (nheA), all the strains possessed enterotoxin FM (entFM). Disc

diffusion method will be used to perform antibiotic susceptibility tests for antibiotics effectiveness against gram-positive bacteria. All test strains show susceptible to doxycycline, oxytetracycline, florfenicol and erythromycin, however all strains show resistant to amoxicillin, ampicillin.