走在汽車革命的路上論文書籍站
國立嘉義大學 農業科學博士學位學程 詹昆衛所指導 徐筠修的 鉤端螺旋體及野生動物肉品鑑定技術開發 （2020），提出EA888 Mobile01關鍵因素是什麼，來自於鉤端螺旋體、環型恆溫增幅法、限制性片段長度多態性、野生動物肉、重組酶聚合酶增幅法。
而第二篇論文高雄醫學大學 公共衛生學系環境暨職業安全衛生博士班 陳培詩所指導 顏昱娟的 家戶空氣汙染物與孩童呼吸道健康 （2019），提出因為有 室內空氣汙染物、呼吸道健康、孩童、工業城市、空氣內毒素的重點而找出了 EA888 Mobile01的解答。
鉤端螺旋體及野生動物肉品鑑定技術開發
為了解決EA888 Mobile01 的問題，作者徐筠修 這樣論述：
鉤端螺旋體病是由鉤端螺旋體菌造成的一種全球性人畜共通傳染病。在台灣，鉤端螺旋體病為一地方性疾病，其發生率在颱風季節 (主要為七、八月份) 時常有成長的趨勢。台灣居民、流浪犬、牛隻、豬隻、小型哺乳類與嚙齒類動物，皆曾進行鉤端螺旋體發生率之研究，唯獨貓隻鉤端螺旋體感染情況尚無紀錄，因此本研究調查了台灣南部貓隻鉤端螺旋體的感染情況，共採集南台灣 233 隻貓隻（159 隻流浪貓與 74 隻家貓），其中 9.3%血清抗體呈現陽性反應 (21隻)；分子檢測結果，血清核酸陽性為 19.1% (25隻)，尿液核酸陽性為 67.8% 隻 (80隻)。為了加速鉤端螺旋體的檢測與分型，本研究應用環形核酸增幅法（
loop mediated isothermal amplification; LAMP）增幅鉤端螺旋體的 lipL32 片段，並使用聚合酶鏈鎖反應結合限制片段長度多態性（polymerase chain reaction-restriction fragment length polymorphism; PCR-RFLP），進行鉤端螺旋體菌之分型。lipL32 LAMP 的診斷敏感性與特異性分別為 91.67% 和 100%， 其不僅具有高度敏感性與特異性，對反應條件也相對較不要求，因此非常適合於資源不足之國家或缺乏設備的現場使用。本篇研究所發表之 PCR-RFLP ，共可區分出六株不同基因
型的鉤端螺旋體，本法不僅可作為鉤端螺旋體病之初步篩檢測驗，也可將環境中或臨床上分離出的菌種進行分型。本論文另一研究主題為建立肉品來源之鑑定方法，並提供肉品稽查人員於現場使用。針對常見被非法食用的台灣野生動物，包括台灣長鬃山羊、台灣山羌、台灣白鼻心、台灣獼猴與台灣穿山甲，共建立五套結合重組酶聚合酶增幅法（recombinase polymerase amplification; RPA）與側流試紙（lateral flow strip; LF strip）的肉品檢測系統，此系統可有效增幅生肉與各種烹煮熟肉的核酸，並與非目標物種無交叉反應。為了讓 RPA-LF 檢測系統可在現場使用，作者也利用行動
電源與 USB 咖啡加熱器，建構出一攜帶式加熱平台。整個肉品的 RPA-LF 檢測，從 DNA 萃取增幅到結果判讀，不需要任何複雜昂貴之機器設備，可在現場使用，且於 20 分鐘內完成，此 RPA-LF 檢測系統，不僅敏感性與特異性皆高，且操作簡單，讓非專業人員可於稽查現場，駕輕就熟的使用本技術鑑定肉品種類來源。
家戶空氣汙染物與孩童呼吸道健康
為了解決EA888 Mobile01 的問題，作者顏昱娟 這樣論述：
TABLE OF CONTENTS1. Introduction…………………………………………………………...12. Objective of this study………………………………………………..53. Research Framework…………………………………………………64. Jumping on the Bed and Associated Increases of PM10, PM2.5, PM1, Airborne Endotoxin, Bacteria, and Fungi Concentrations……………74.1 Introduction……………
………………………………………………………….74.2 Materials and Methods……………………………………………………………84.2.1 The simulation of jumping on the bed and making the bed…………………….84.2.2 Instruments and sample processing……………………………………………..94.2.3 Analysis of airborne bacteria, fungi, and endotoxin…………………………….94.2.4 Ethics………………………………………
…………………………………...104.2.5 Statistical analyses……………………………………………………………...104.3 Results……………………………………………………………………………114.4 Discussion………………………………………………………………………..134.5 Conclusion……………………………………………………………………….175. Cooking/ Window opening and associated increases of indoor PM2.5 and NO2 concentrations of ch
ildren’s houses in Kaohsiung, Taiwan…...335.1 Introduction………………………………………………………………………335.2 Materials and Methods…………………………………………………………...345.2.1 Study Area……………………………………………………………………...345.2.2 Study Design…………………………………………………………………...345.2.3 Air Sampling…………………………………………………………………...355.2.4 Household Ch
aracteristics……………………………………………………...355.2.5 Ethics…………………………………………………………………………...365.2.6 Statistical Analyses……………………………………………………………..365.3 Results……………………………………………………………………………365.4 Discussion………………………………………………………………………..385.5 Conclusions………………………………………………………………………416. Household airborne endotoxin
association with asthma and allergy in elementary-school-age children: A case-control study in Kaohsiung, Taiwan…………………………………………………………………...476.1 Introduction……………………………………………………………476.2 Materials and Methods…………………………………………………………...486.2.1 Study design and population…………………………………………………...486.2.2 H
ome visit……………………………………………………………………...486.2.3 Home characteristics…………………………………………………………...496.2.4 Sampling and analysis of airborne endotoxin………………………………….496.2.5 Bacterial and fungal bioaerosols……………………………………………….506.2.6 Statistical analysis……………………………………………………………...506.3 Results………………………………………………
……………………………516.4 Discussion………………………………………………………………………..516.5 Conclusion……………………………………………………………………….537. Indoor Ozone and Particulate Matter Modify the Association between Airborne Endotoxin and Schoolchildren's Lung Function………………667.1 Introduction………………………………………………………………………667.2 Materials and Meth
ods…………………………………………………………...677.2.1 Study Design…………………………………………………………………...687.2.2 Study subjects…………………………………………………………………..687.2.3 Outcome measurements………………………………………………………..697.2.4 Exposure assessment, analysis, and instrument calibration……………………697.2.5 Statistical Analyses……………………………………………………………
..707.3 Results……………………………………………………………………………717.3.1 The descriptive statistics of airborne endotoxin and air pollutants…………….717.3.2 The characteristics and lung function of study subjects………………………..717.3.3 Interaction between airborne endotoxin and indoor air pollutants……………..727.4 Discussion………………
………………………………………………………..727.5 Strength and limitation…………………………………………………………...757.6 Conclusion………………………………………………………………………..758. The correlation between household air pollutants and schoolchildren's asthma and lung functions in an industrial city: a case-control study………………………………………………………..838.1 Intr
oduction………………………………………………………………………838.2 Materials and Methods………………………………………………………….848.2.1 Study design……………………………………………………………………848.2.2 Study subjects…………………………………………………………………..848.2.3 Outcome measurements………………………………………………………..858.2.4 Exposure assessment, analysis, and instrument calibration………
……………858.2.5 Statistical Analyses……………………………………………………………..868.3 Results……………………………………………………………………………868.4 Discussion………………………………………………………………………..878.5 Conclusion………………………………………………………………………..909. Early exposure of household airborne endotoxin and development of respiratory health……………………………………………………
…...9710. Summary and suggestions………………………………………….100Reference……………………………………………………………………………101 LIST OF FIGURESFig 4.1. The flowchart of sampling time……………………………………………..31Fig 4.S1. The trend of PM concentrations between beating the bed and jumping on the bed……………………………………………………………………………………32Figure 5.
1. The 24-h average concentration of indoor air pollutants (A) CO, (B) CO2, (C) NO2, and (D) O3 in the houses of traffic, industry, and general areas…………...45Figure 7.S1: The set up of air sampling……………………………………………...82Figure 9.1 the calibration curve of endotoxin with R2 of 0.994……………………...99 LIST
OF TABLESTable 4.1. The household characteristics in 60 children’s houses……………………18Table 4.2. Descriptive statistics of PM, bioaerosols and environmental factors during the period of jumping on the bed, making the bed, and background assessment……19Table 4.3. The increased concentrations of PM10, PM2
.5, PM1, and airborne bacteria, fungi and endotoxin, when jumping on the bed and making the bed paired with background measurements from the same house…………………………………….20Table 4.4. The ratio of jumping on the bed and making the bed to background for PM10, PM2.5, PM1, airborne bacteria, fungi and endot
oxin when jumping on the bed and making the bed paired with background measurements from the same house….21Table 4.5. Correlation between pollutants when jumping on the bed, making the bed, and background………………………………………………………………………22Table 4.6. Association between PM, airborne bacteria, fungi and end
otoxin concentrations (dependent variable), and household characteristics during the background: Robust regression analysis……………………………………………..24Table 4.7. Association between PM, airborne bacteria and fungi concentrations (dependent variable), and household characteristics when jumping on the bed: R
obust regression analysis, adjusting temperature, relative humidity, and background concentration…………………………………………………………………………27Table 4.8. Association between airborne bacteria and fungi concentrations (dependent variable), and household characteristics when making the bed, and between airborne endot
oxin concentrations (dependent variable), and household characteristics during jumping on the bed and making the bed: Robust regression analysis, adjusting temperature, relative humidity, and background concentration……………………...29Table 5.1. Descriptive statistics of 24-h average indoor and outdoor ai
r pollutants, temperature, and relative humidity in 60 houses……………………………………..42Table 5.2. The percentage (%) of household characteristics in traffic, industry, and general areas………………………………………………………………………….42Table 5.3. The ratios of air pollutants during window opening periods to reference periods a
nd the differences in air pollutants between window opening periods and reference periods……………………………………………………………………..43Table 5.4. The ratios of air pollutants during cooking periods to reference periods and the differences in air pollutants between during cooking periods and reference periods…………………
……………………………………………………………..43Table 5.5. Association between air pollutants concentrations (24-h average concentration of air pollutants in each house as dependent variable), and household characteristics: generalized estimating equations……………………………………44Table 5. S1. Descriptive statistics of 24-hr aver
age concentration of indoor air pollutants in the houses of traffic, industry and general areas……………………….46Table 6.1. Descriptive statistics of airborne endotoxin, bacteria, and fungi concentration, temperature and relative humidity……………………………………54Table 6.2. Odds ratios (ORs) for association of airb
orne endotoxin with respiratory disease and symptoms………………………………………………………………..55Table 6.3. Odds ratios (ORs) for association of airborne bacteria with respiratory disease and symptoms………………………………………………………………..57Table 6.4. Odds ratios (ORs) for association of airborne fungi with respiratory disease a
nd symptoms………………………………………………………………………..59Table 6.S1. Characteristics of the school-children of case-control groups (N=120)...61Table 6.S2. Airborne endotoxin, bacteria, and fungi concentration, temperature, and relative humidity of case and control group………………………………………….63Table 6.S3. Association be
tween bioaerosols and the environmental parameters: the robust regression analysis……………………………………………………………64TABLE 7.1. DESCRIPTIVE STATISTICS OF INDOOR AIRBORNE ENDOTOXIN AND INDOOR AIR POLLUTANTS………………………………………………..76TABLE 7.2. THE CORRELATION BETWEEN INDOOR AIRBORNE ENDOTOXIN AND INDOOR AIR POLLUTA
NTS………………………………..77TABLE 7.3. CHARACTERISTICS OF THE STUDY SUBJECTS…………………78TABLE 7.4. THE ASSOCIATIONS BETWEEN AIRBORNE ENDOTOXIN AND SCHOOLCHILDREN’S LUNG FUNCTION……………………………………….79TABLE 7.5. THE EFFECTS OF INDOOR O3 CONCENTRATION ON ASSOCIATIONS BETWEEN AIRBORNE ENDOTOXIN AND LUNG FUNCTION…………………
………………………………………………………..80TABLE 7.6. THE EFFECTS OF INDOOR PM10 CONCENTRATION ON ASSOCIATION BETWEEN AIRBORNE ENDOTOXIN AND LUNG FUNCTION…………………………………………………………………………..81Table 8.1. Descriptive statistics of indoor and outdoor air pollutants concentration at homes in asthmatic children, and non-asthmat
ic children…………………………...91Table 8.2a. Descriptive statistics of lung functions on day 1 in asthmatic children, and non-asthmatic children……………………………………………………………….92Table 8.2b. Descriptive statistics of lung functions on day 2 in asthmatic children, and non-asthmatic children (Continued)…………………………………
……………….93Table 8.3. Analysis of coefficient of stepwise regression models (The difference in lung function between Day 1 and Day 2 as dependent variable)…………………….95Table 8.4. Association between indoor air pollutants concentrations and schoolchildren’s lung functions: Multiple regressions……………………………
….96