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薑黃於降低角膜內皮細胞氧化壓力之研究

為了解決model 3 sr 0-100的問題，作者郭曉佩 這樣論述：

Fuchs'角膜內皮細胞失養症是最常見的角膜內皮細胞失養症，也是角膜移植的主要適應症。在這些患者的角膜內皮細胞中觀察到抗氧化功能的受損。 許多天然植物可以增強細胞的抗氧化功能，其中之一就是薑黃素。之前沒有相關研究討論薑黃素在角膜內皮疾病的治療作用，因此我們主要研究目是探討薑黃素是否有利於角膜內皮細胞在氧化壓力下存活。將不同濃度 (0-100 μM) 和不同治療期 (24 小時vs 48 小時) 的薑黃素施加於人類角膜內皮細胞的細胞株 (B4G12)。 氧化壓力的誘發則藉由施加2 小時 0.25 mM 第三丁基過氧化氫 (t-BHP)。細胞的存活、增殖和分化表型會加以檢驗。藉由流式細胞儀和 C

CK-8 可分別評估活性氧物質 (ROS) 的產生和細胞存活。透過免疫熒光分析角膜內皮細胞膜上蛋白的表達可以知道實驗中細胞結構是否被破壞。而 Keap-1、細胞中Nrf-2、細胞核內 Nrf-2的表現及Nrf-2相關路徑的產物則藉由西方墨點法檢測。實驗結果證實，預先給予薑黃素對於細胞後續受到氧化壓力時的細胞存活有顯著幫助。預先給予24小時不同濃度的薑黃素對細胞存活皆有幫助（1.5、3.125、6.25、12.5 和 25 μM），特別是在薑黃素濃度 12.5 μM 時，角膜內皮細胞呈現出典型六邊形形態和優異的存活率。 在預先給予薑黃素的組別，ROS的產生有顯著降低。此外，免疫熒光顯示有先暴露於

薑黃素的細胞即使遭受氧化壓力， zonula occludens-1 的膜蛋白表現比無預先施加薑黃素的組別要好。西方墨點法證實薑黃素的施加可以提高細胞核內Nrf2的信號。除此之外，預先暴露於薑黃素可以增強細胞受到氧化壓力時Keap1/Nrf2/ARE 路徑的產物（超氧化物歧化酶SOD-1 和血紅素加氧酶HO-1）的表現。我們的研究結果顯示，薑黃素可以增強角膜內皮細胞遭受氧化壓力時的存活和分化。薑黃素對Keap1/Nrf2/ARE 路徑的活化為薑黃素可增強細胞抗氧化能力的機制之一。 由於眼藥水或前房注射的便利性，這一發現可能有助於薑黃素在角膜內皮疾病中找到新的治療角色

Upconversion Nanohybrids for Intracellular pH Imaging and Broadband Photodetection

為了解決model 3 sr 0-100的問題，作者高聖禹 這樣論述：

Nanohybrids or nanocomposites (NC) offer a wider scope in materials engineering andapplications by utilizing the extended range properties of the individual component materials,and those from the novel interfacial properties. An intriguing aspect of such NC is the chargetransfer that happens among

the components. Synthetic techniques are plenty; however,choosing the correct combination for the NC is important depending on target applications,such as intracellular pH sensing and photodetection, in particular. Previously reportedfluorescent nanoprobes for intracellular pH sensing had drawbacks,

such as shallow penetrationdepth of the excitation light, background autofluorescence, poor photostability, andbiocompatibility. On the other hand, pure or single material-based photodetector devices,although having a fast response, either lacked broadband response, have poorphotoresponsivity, or b

oth. Through this thesis, we have attempted to design and synthesizetwo novels NC of upconversion nanoparticles (UCNPs): i) with mOrange (mO) fluorescentprotein (FP) for fluorescence-based intracellular pH sensing; ii) with molybdenum disulfide(MoS2) for ultrasensitive broadband photodetection, that

overcomes the above-mentionedissues.First, we have constructed a Forster Resonance Energy Transfer (FRET) based pH nanoprobeutilizing the charge transfer between the UCNP donor and the mOrange FP acceptor forintracellular pH sensing. The UCNP-mOrange nanoprobes (on a coverslip or uptaken in asingle

HeLa cell) could be fluorescently imaged with 980 nm excitation. The FRET probesshow FRET efficiency of ~20% at pH = 7.0 and show pH-sensitive simultaneous selfratiometric and ratiometric features. Nigericin-mediated intracellular pH (3.0, 5.0, and 7.0)could be accurately estimated from the fluores

cence-derived FRET ratio. The nanoprobeiiiexhibits good accuracy, reversibility, and stability over a wide range of pH (3.0–8.0), eveninside a cell. The fluorescence intensity ratio from UCNP and mOrange could be used toestimate the pH inside a single HeLa cell.In the second case, we have fabricated

a photodetector (PD) device with a single flake of MoS2electrostatically conjugated with the UCNPs. The idea was to extend the conventional workingrange of the MoS2, within 200-680 nm, to the near-infrared (NIR) regime. The device wasirradiated with power-dependent 325-1064 nm illumination to study

its broadbandphotosensitivity. The highest responsivity of 1254 A W-1 is reported for 980 nm at 1.0 V bias.An unprecedented normalized gain of 7.12 x 104 cm2 V-1, and Detectivity of 1.05 x 1015 Jones(@980 nm, 1 V) was obtained. The real application of the PD device was demonstrated usingnon-laser d

omestic appliances such as sodium vapor lamp, mobile phone flashlight, and aircondition remote controller.