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Without Restraint: How Skiing Saved My Son’s Life

為了解決Antarctica的問題，作者Delena, Robert C.,Delena, Ryan C. 這樣論述：

As a child Ryan DeLena had difficulty controlling his emotional outbursts. This led to placement in therapeutic schools that relied on detrimental methods of behavior modification such as physical restraints. Nothing helped from a team of doctors to heavy medication. Then in 2010, Ryan was voluntari

ly committed to a mental hospital for further evaluation. His parents Rob and Mary Beth were counseled to place him in a group home. They refused. Two years earlier, after an impulsive decision Rob had to take Ryan skiing, he discovered a different child than the version experts were so sure about.

By his second day of skiing, Ryan was executing advanced runs, and with each conquest in the winters that followed, Rob began to question the path laid for his son by the professionals paid to judge him. He later convinced Mary Beth to fight the medical and educational complexes over Ryan’s care and

school placement, and together they fostered the freedom Ryan needed to pursue his dream of becoming a professional ski mountaineer. Written in two voices, Without Restraint is a joint father-son memoir told with both pain and levity, struggle and strength, adventure and heart. It is the story of a

misunderstood boy, a father’s growth, and a shared love of the outdoors that formed their unbreakable bond. Robert C. DeLena was raised in Revere, MA, and is a graduate of The Governor’s Academy, Trinity College, and Northeastern University School of Law. After practicing law unhappily, he founde

d a small recruiting company called Legal Staffing Solutions, and for over twenty years has advised law firms, lawyers, and law students on legal hiring. Rob lives in Sudbury, MA with his wife, Mary Beth, and his daughter, Abigail, who currently attends Hamilton College. He spends time skiing with h

is son Ryan and the great friends he’s made during his journey from beginner to reluctant adventurer. Rob has skied all over the United States, internationally in Canada, Chile and Argentina, and even survived a backcountry expedition in Antarctica. He is planning to return to Antarctica with Ryan i

n late 2022. Ryan C. DeLena is currently a junior at Northern Vermont University studying Outdoor Education. He is widely known in the outdoor community through his social media presence as ＂Extreme Ryan.＂ He was pictured on the cover of Backcountry Ski Maps (2020) and has conquered many of the worl

d’s signature ski runs including Super-C Couloir in Chile, Little Couloir in Montana, and Tuckerman Ravine in New Hampshire. Ryan has climbed and skied additional peaks in Oregon, Washington, Utah, California, Nevada, Wyoming, and Antarctica. He is an enthusiastic rock climber, ice climber, and avid

hiker, summiting the Grand Teton twice and has recently completed the ＂Hundred Highest＂ hiking peaks in New England. Ryan has earned advanced certifications from the American Mountain Guides Association and the Professional Ski Instructors of America. He spends every available moment in the White M

ountains and plans on adventuring in Svalbard, Peru, and Antarctica in 2022.

Antarctica進入發燒排行的影片

蠶蛾科之起源、演化及其生物學研究

為了解決Antarctica的問題，作者林容諄 這樣論述：

馴化已久的家蠶(Bombyx mori)(Lepidoptera: Bombycidae)，是鱗翅目的模式物種之一，為第一個完成全基因組序列定序(genome sequence)的鱗翅目物種。由於家蠶結繭的蠶絲及蠶絲的副產品擁有重要的經濟價值，根據文獻記載早在5000年前就開始被人類馴養利用，目前有超過1000種以上的品系。近年結合分子數據證據透過馴化品系之間的基因組關係研究顯示，家蠶(B. mori)是由野蠶(B. mandarina)所馴養而來，與歷史文獻紀錄吻合，透過絲路分別傳往世界各地。儘管家蠶研究深入，然而蠶蛾科內其他與蠶蛾近緣物種的研究資料十分缺稀，例如多數的蠶蛾科物種沒有完整的

生活史資料，蠶蛾科內不同屬之間的關係尚未釐清，幼蟲取食桑科(Moraceae)桑屬(Morus)或榕屬(Ficus)植物的演化情形未知，且蠶蛾科的分布起源仍然充滿疑問。本研究取樣所有文獻記錄地區之蠶蛾科樣本，囊括亞洲、南美洲、非洲、澳洲及東南亞地區，研究結果簡述如下：1) 採集、飼養蠶蛾科物種、記錄其生活史資料包含寄主植物、卵的排列方式、初齡幼蟲之原生毛序、繭的結構與色型等；2) 以6個基因(1個粒線體基因與5個核基因)序列資料建構可靠的蠶蛾科親緣關係，結果顯示蠶蛾科可分為南美洲新世界亞科Epiinae與亞洲古北區舊世界亞科Bombycinae兩個亞科，屬級關係中除Bivincula與Gund

a為併系群，其餘屬為單系群；3) 根據幼蟲的寄主植物記錄與分子數據建構的親緣關係樹進行特徵演化模擬，推測利用桑科榕屬做為寄主植物為祖徵，而利用桑科桑屬植物則為較晚近演化出來的結果；4) 利用新建構之分子親緣關係樹結合化石、地質年代與分子鐘估算物種分化時間，重建蠶蛾科之生物地理歷史事件，估算蠶蛾科約在6千1百萬年前於岡瓦那大陸(Gondwana)出現，約在5千1百萬年前分化為新世界與舊世界兩大亞科；分析顯示，Bombycinae的祖先約在4千6百萬年前經由一次澳洲至亞洲的擴散事件(dispersal)形成現今物種在東南亞及亞洲的分布；而後又再經由一次的擴散事件，從亞洲擴散至非洲地區。

The Land Beneath the Ice: The Pioneering Years of Radar Exploration in Antarctica

為了解決Antarctica的問題，作者Drewry, David J. 這樣論述：

A wondrous story of scientific endeavor--probing the great ice sheets of AntarcticaFrom the moment explorers set foot on the ice of Antarctica in the early nineteenth century, they desired to learn what lay beneath. David J. Drewry provides an insider’s account of the ambitious and often hazardou

s radar mapping expeditions that he and fellow glaciologists undertook during the height of the Cold War, when concerns about global climate change were first emerging and scientists were finally able to peer into the Antarctic ice and take its measure. In this panoramic book, Drewry charts the hist

ory and breakthrough science of radio-echo sounding, a revolutionary technique that has enabled researchers to measure the thickness and properties of ice continuously from the air--transforming our understanding of the world’s great ice sheets. To those involved in this epic fieldwork, it was evide

nt that our planet is rapidly changing, and its future depends on the stability and behavior of these colossal ice masses. Drewry describes how bad weather, downed aircraft, and human frailty disrupt the most meticulously laid plans, and how success, built on remarkable international cooperation, ca

n spawn institutional rivalries. The Land Beneath the Ice captures the excitement and innovative spirit of a pioneering era in Antarctic geophysical exploration, recounting its perils and scientific challenges, and showing how its discoveries are helping us to tackle environmental challenges of glob

al significance.

利用CRISPR/Cas9技術置換熱休克蛋白104之啟動子以提高釀酒酵母Kyokai No.7之高溫發酵能力

為了解決Antarctica的問題，作者陳婷依 這樣論述：

謝辭 i摘要 iiAbstract iv圖目錄 xi表目錄 xiii附錄圖目錄 xiv縮寫表 xv胺基酸表 xvi第一章 緒論 11.1 前言 11.2 生物乙醇 11.3 釀酒酵母 21.4 釀酒酵母的代謝途徑 21.5 環境因子對酵母菌的抑制作用 61.6 熱休克蛋白（Heat shock protein） 71.6.1 熱休克蛋白 104（HSP104）的結構 91.6.2 熱休克蛋白 104 功能與調節 111.7 CRISPR/Cas9 技術 111.7.1 Cas9 蛋白 151.7.2 引導 RNA 151.7.3 供體 DNA 161.7.4 CRISPR 基因編輯效率 16

1.8 研究動機 171.9 實驗設計 19第二章 藥品與設備 222.1 實驗儀器 222.2 實驗器材 232.3 培養基 242.4 菌種與質體 242.5 抗生素 242.6 質體純化試劑 242.7 PCR 藥品 252.8 DNA 改殖藥品 252.9 DNA 電泳試劑 252.10 酵母菌轉型藥品 262.11 其他藥品 26第三章 材料與研究方法 273.1 抗生素 273.1.1 Ampicillin stock solution (100 mg/mL) 273.1.2 G418 stock solution (100 mg/mL) 273.2 50% ( W/V ) 葡萄

糖溶液 273.3 培養液 283.3.1 Luria-Bertani (LB) 培養液 283.3.2 LB agar plate (抗 Ampicillin) 283.3.3 Yeast extract peptone dextrose medium(YPD medium) 283.3.4 YPD agar plate (抗 G418) 283.4 電穿孔轉型相關藥品 293.4.1 1 M Sorbitol 293.4.2 1 M Lithium acetate 293.4.3 10x Tris-EDTA buffer 293.4.4 1 M DTT 293.5 菌體培養 303.5.1

大腸桿菌（Escherichia coli；E. coli）培養 303.5.2 酵母菌培養 303.5.3 菌種保存 303.6 質體建構相關步驟 303.6.1 E. coli 質體純化 303.6.2 限制酶處理(Restriction enzyme digestion) 313.6.3 DNA 電泳 323.6.4 引子（primer）稀釋 323.6.5 Polymerase chain reaction(PCR) by KOD hot start polymerase 323.6.6 Polymerase chain reaction(PCR) by Taq polymeras

e 343.6.7 限制酶 DpnI 去除甲基化的質體 363.6.8 DNA cleaning(使用 Zymo DNA Clean & Concentrator™-5) 363.6.9 Gel Extraction(使用 Zymo DNA Clean & Concentrator™-5 &Gene-Spin™ 1-4-3 DNA Purification Kit - V3) 373.6.10 Gibson 組裝 DNA 片段 373.6.11 大腸桿菌質體轉型(Transformation) 383.7 建構耐高溫酵母菌之相關步驟 383.7.1 酵母菌電穿孔法轉型(Transformati

on) 383.7.2 Colony PCR 39第四章 結果與討論 414.1 E. coli 相關質體構建 414.1.1 pML104-KanMX 質體純化 414.1.2 構建 pML104-KanMX-Target1 424.1.3 構建 pML104-KanMX-Target2 464.1.4 pTEF1-2m-ori-HSP26-isa1-40homo 質體純化 494.1.5 利用 PCR 將 40homo-FRT-HSP26p-40homo 片段放大 514.2 構建耐高溫釀酒酵母 Kyokai No.7-HSP26P 534.2.1 轉型結果 534.2.2 Kyokai

No.7-HSP26P 之 Colony PCR 結果 54第五章 未來展望 57參考文獻 58附錄 65