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製造奈米結構式電極應用於即時檢測活體細胞內氧化還原型信號分子

為了解決DAF CF的問題，作者Nithiya Jeromiyas 這樣論述：

The combination of nanomaterials in electrochemical sensors are an attractive approach to improving sensitivity and can also provide improved stability and selectivity.In this report, the new trend and the current state of the art of nanostructure-based on electrochemical detection of endogenous pr

oduction of hydrogen peroxide (H2O2) and hydrogen sulfide (H2S) from live cells. A series of three nanostructured electrodes ZnCo2O4/Co3O4/CF, POPD/RGO-MoS2, and POPD/ Laser-scribed graphene based on their selective and anti-biofouling properties were designed to specifically target and quantify end

ogenous H2O2, H2S, and H2S releasing profiling of anti-cancer drugs. The proposed electrochemical platform involves four crucial steps started from 1.Design and Synthesis of nanostructured material, 2. Material characterization, electrode fabrication, 3.Proof-of-concept, and electrocatalytic determi

nation of lab samples, 4. Examine the real-time monitoring and quantification of biological fluids such as fetal bovine serum, human serum, human blood, and live cells. The ZnCo2O4/Co3O4/CF promotes H2O2 reduction at minimized overpotential (−0.10 V vs Ag/AgCl). which is several millivolts away fro

m the voltammetric regions of common biological and oxygen interferences, and highly selective in the presence of 5-fold excess concentrations of biological species. In further that electrode endows ultrasensitivity (detection limit = 1 nM) and quantifies the amount of H2O2 released from RAW 264.7 (

8.7 × 10−14 mol).For hydrogen sulfide detection, sulfur passivation, electrode poisoning, and interferences from complex biological environments are major challenges in the development of in-situ H2S sensors. The POPD/RGO-MoS2-modified electrode catalyzed H2S oxidation at a minimized overpotential (

+0.15 V vs. Ag/AgCl). The RGO-MoS2 with o-phenylenediamine (POPD) electropolymerized films were characterized for their H2S permselective behavior against common biological interferents. Moreover, this method displayed a detection limit of 10 nM, which covers the endogenous H2S levels. Further real-

time monitoring of H2S production demonstrated in live Escherichia MG1655 and quantification of H2S spiked in FBS human serum, and human blood samples accurately quantified with excellent practical feasibility. In addition H2S is involved in biological processes of cancer and H2S has involves both p

ro-cancer and anti-cancer effects. H2S-releasing donors exhibit anti-cancer effects. The POPD/Laser-scribed graphene electrode has been designed for the in-situ profiling of H2S and a handy analytical accessory for cancer therapy. The POPD/Laser-scribed graphene (LSG) electrode delivered an outstand

ing electrocatalytic performance of point-of-care H2S sensing with a detection limit of 1 nM. In further, POPD/Laser-scribed graphene has been employed in situ real-time tracking of H2S monitoring and the H2S releasing profile of anti-cancer drugs from live cells as early diagnostic strategies.